Abstract
Sensitivities of seven commonly used bioassays for Helminthosporium maydis race T-toxin were determined. The most easily quantified and highly sensitive bioassays were based either on dark CO 2 fixation by green leaves or on respiration rates of isolated mitochondria. These bioassays were 5 to 10 times more sensitive than was the semiquantitative seedling root growth bioassay. Bioassays based on the production of visible lesions on leaves did not provide reliable dosage-response relationships and were estimated to be 20 to 100 times less sensitive to toxin than dark CO 2 fixation or mitochondrial respiration. Choice of bioassay depends on the objective of the experiment in which it is used.
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