Abstract
AbstractA highly sensitive and specific liquid chromatography‐elctrospray ionization tandem mass spectrometry method was developed and validated for rapid determination of risperidone and its active metabolite in rat dried blood spots and dried plasma spots. Chromatographic separation was achieved through ZIC®‐HILIC (250 × 4.6 mm, 5 µm) at 25°C using 10 mM ammonium acetate (pH: 3.0, adjusted with acetic acid) and acetonitrile (12:88, v/v) as a mobile phase. Tandem mass spectrometry detection was performed in positive ion electrospray ionization using multiple reaction monitoring mode to monitor precursor → product ion transitions m/z 411.2→191.1 for Risperidone, m/z 427.2→207.1 for its metabolite (Risperidone‐9‐OH), respectively. The lower limit of quantification of Risperidone and Risperidone‐9‐OH were 0.95 and 0.90 ng/mL in rat dried blood spots, respectively. The developed bioassay method was successfully applied to study the pharmacokinetic profile of Risperidone and Risperidone‐9‐OH using Iloperidone as an internal standard. As per pharmacokinetic studies, Cmax of Risperidone and Risperidone‐9‐OH in dried blood spots was 36.2 and 28.9 ng/mL; and in dried plasma spots was 38.2 and 30.2 ng/mL, respectively. Similarly, tmax of Risperidone and Risperidone‐9‐OH in dried blood spots was 1.4 and 14.7 h; and in dried plasma spots was 1.54 and 15.4 h, respectively.
Published Version
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