Abstract
The present work constitutes a contribution to the phytochemical investigation of Cryptoporus volvatus aiming to search for effective cytotoxic constituents against tumor cell lines in vivo. Bioassay-guided separation of the ethylacetate extract of C. volvatus afforded four new isocryptoporic acid (ICA) derivatives, ICA-B trimethyl ester (1), ICA-E (2), ICA-E pentamethyl ester (3), and ICA-G (4), together with nine known cryptoporic acids. These isocryptoporic acids are isomers of the cryptoporic acids with drimenol instead of albicanol as the terpenoid fragment; their structures were elucidated on the basis of spectroscopic evidences (UV, IR, HRMS, and NMR) and comparison with literature values. All isolates show certain cytotoxic activities against five tumor cell lines. Among them, compound 4 showed an comparable activity to that of the positive control cis-platin, while other compounds exhibited weak cytotoxic activities.
Highlights
Cryptoporus volvatus (Pk.) Hubbard, belonging to the Polyporaceae, grows on living trees or dead wood
In order to find other constituents with higher cytotoxic activities, we examined the fruiting bodies of C. volvatus by the bioassay-guided separation, which led to the isolation of four new chemical components as well as nine known components
Compound 1 was obtained as a yellow oil and the molecular formula was established by MALDI-TOF/TOF-MS as C24 H38 O8, with a quasi-molecular ion peak of [M + Na]+ at m/z 477.2464
Summary
Cryptoporus volvatus (Pk.) Hubbard, belonging to the Polyporaceae, grows on living trees or dead wood. The fruiting bodies of this fungus have long been used for the treatment of asthma and bronchitis in Chinese traditional medicine [1]. Derivatives have been isolated from this plant [2,3,4,5]. In order to find other constituents with higher cytotoxic activities, we examined the fruiting bodies of C. volvatus by the bioassay-guided separation, which led to the isolation of four new chemical components as well as nine known components (CA-A, CA-A trimethyl ester, CA-B, CA-B trimethyl ester, CA-C, CA-C pentamethyl ester, CA-D, CA-D pentamethyl ester, and CA-E pentamethyl ester). The isolation, structural elucidation, and cytotoxic properties towards five human cancer cell lines of all fractions and isolates were reported
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
