Abstract
Tomato root exudates were analyzed using a bioassay to detect the chemoattractant for Ralstonia solanacearum. An activated charcoal-adsorbed fraction of root exudates from tomato cultivar Oogata-fukuju had chemoattractant activity for R. solanacearum strain MAFF 730138. The active component, purified using a Sep-Pak C18 cartridge, an activated charcoal column, diol-modified silica gel, and NH2-modified silica gel, is a new hydrophobic attractant. The final purified fraction produced a single peak in a diol-modified silica gel HPLC analysis.
Highlights
Bacterial wilt caused by Ralstonia solanacearum (Yabuuchi et al 1995) is one of the most devastating bacterial plant diseases in tropical, subtropical, and warm temperate regions worldwide (Hayward 1991)
The objective of this study was to identify the substance(s) in tomato root exudates that are attractive to R. solanacearum using a bioassay-guided approach
A 20-μL aliquot of the bacterial suspension was placed onto the center of a Petri dish (6 cm i.d.) containing 2.8 mL semisolid medium
Summary
Bacterial wilt caused by Ralstonia solanacearum (Yabuuchi et al 1995) is one of the most devastating bacterial plant diseases in tropical, subtropical, and warm temperate regions worldwide (Hayward 1991). R. solanacearum is a soil-borne Gram-negative bacterium that infects more than 200 plant species from 50 families, including economically important crops such as tomato, potato, eggplant, pepper, tobacco and banana (Elphinstone 2005). This bacterial plant pathogen can survive long periods in water, soil, and latently infected plants (Álvarez et al 2008). It can be transmitted via soil, water, farm equipment, and infected plant materials. Because of its wide geographic distribution, extensive host range, and tolerance to various environmental conditions, R. solanacearum is one of the most destructive crop pathogens worldwide
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