Bioassay-Guided Evaluation of Antinociceptive Effect ofN-Salicyloyltryptamine: A Behavioral and Electrophysiological Approach

Lucindo J Quintans-Júnior,Valter J Santana-Filho,Reinaldo N Almeida,Adriano A S Araújo,Jullyana S Siqueira,Davi A Silva,Leonardo R Bonjardim,Josimari M Desantana,Rosana S S Barreto,Stanley J C Gutierrez,Maria F V Souza,Demétrius A M Araújo,Waldeci De Lucca Júnior,José Maria Barbosa-Filho

doi:10.1155/2010/230745

Abstract

We investigated the antinociceptive and nerve excitability effects of the N-salicyloyltryptamine (NST) NST-treated mice exhibited a significant decrease in the number of writhes when 100 and 200 mg/kg (i.p.) were administered (i.p.). This effect was not antagonized by naloxone (1.5 mg/kg, i.p.). NST inhibited the licking response of the injected paw when 100 and 200 mg/kg were administered (i.p.) to mice in the first and second phases of the formalin test. Because the antinociceptive effects could be associated with neuronal excitability inhibition, we performed the single sucrose gap technique and showed that NST (3.57 mM) significantly reduced (29.2%) amplitude of the compound action potential (CAP) suggesting a sodium channel effect induced by NST. Our results demonstrated an antinociceptive activity of the NST that could be, at least in part, associated to the reduction of the action potential amplitude. NST might represent an important tool for pain management.

Highlights

In the past few decades, a number of studies have been performed in an attempt to elucidate and understand the factors and mechanisms involved in normal sensory, pain perception and its treatment
Because the antinociceptive effects could be associated with neuronal excitability inhibition, we performed the single sucrose gap technique and showed that NST (3.57 mM) significantly reduced (29.2%) amplitude of the compound action potential (CAP) suggesting a sodium channel effect induced by NST
Our results demonstrated an antinociceptive activity of the NST that could be, at least in part, associated to the reduction of the action potential amplitude

Summary

Introduction

In the past few decades, a number of studies have been performed in an attempt to elucidate and understand the factors and mechanisms involved in normal sensory, pain perception and its treatment. The anticonvulsant drugs are able to reverse or avoid seizures acting by decreasing the neuronal response to seizure-induced stimuli which are commonly used to treat pain disorders [3]. These drugs have a role in its treatment due to the fact that some clinical pain disorders seem to have common physiopathogenic mechanisms with seizures [4]. In this way, phenytoin and carbamazepine have been evidenced to exert

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