Bioassay, Extraction, and Purification Procedures for Wortmannin, the Hemorrhagic Factor Produced by Fusarium oxysporum N17B Grown on Rice

Abstract

Abstract Rats and cultured cells were treated with purified wortmannin toxin, crude fungal extracts, and partially purified preparations for periods of 5-96 h. A single dose of the toxic fractions containing the equivalent of 2.5 g fungus-infested rice resulted in death, hematuria, and hemorrhage in the urinary bladder, Intestine, stomach, heart, and thymus of rats. Purified wortmannin produced the same effects at 4 mg/kg. Fractions that were toxic in vivo also had cytotoxic properties with various cultured cell lines at concentrations ranging from 1.2 to >50 mg equivalents of fungus-infested rice extracted/mL Purified wortmannin was cytotoxic at 50 μg/mL.