Abstract
Objective: The aim of this study was to develop and validate simple and economical HPLC method for estimation of Teriflunomide in human plasma. Methods: HPLC method was developed using AgilentEclipse XBD C8 (4.6 mm×150 mm) as stationary phase and mobile phase used was ammonium acetate buffer: methanol (40: 60 v/v). The detection was carried at wavelength 294 nm. A simple protein precipitation technique was used with acetonitrile as protein precipitating agent and Paliperidone palmitate was chosen as internal standard. Validation was carried out as per USFDA guidelines for bio-analytical method. Results: The linearity range set was 10–60 μg/ml. The value of regression coefficient was found to be 0.9953. Retention time for Teriflunomide was found to be 4.8 min. The developed method was validated for various parameters such as specificity, linearity, accuracy, precision, recovery, and stability. Conclusion: The developed method is simple, rapid, and economical for estimation of Teriflunomide in human plasma.
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More From: Asian Journal of Pharmaceutical and Clinical Research
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