Abstract
Objective: The aim of this study was to develop and validate simple and economical HPLC method for estimation of Teriflunomide in human plasma. Methods: HPLC method was developed using AgilentEclipse XBD C8 (4.6 mm×150 mm) as stationary phase and mobile phase used was ammonium acetate buffer: methanol (40: 60 v/v). The detection was carried at wavelength 294 nm. A simple protein precipitation technique was used with acetonitrile as protein precipitating agent and Paliperidone palmitate was chosen as internal standard. Validation was carried out as per USFDA guidelines for bio-analytical method. Results: The linearity range set was 10–60 μg/ml. The value of regression coefficient was found to be 0.9953. Retention time for Teriflunomide was found to be 4.8 min. The developed method was validated for various parameters such as specificity, linearity, accuracy, precision, recovery, and stability. Conclusion: The developed method is simple, rapid, and economical for estimation of Teriflunomide in human plasma.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Asian Journal of Pharmaceutical and Clinical Research
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
