Abstract
Bioanalytical method Validation employed for quantitative determination of drug and their metabolites in biological fluids. Comprises all criteria determining data quality, such as selectivity, accuracy, precision, recovery and senstivity. The main purpose of method validation is to demonstrate that a specific Bioanalytical method can reliably determine the concentration of drug in study sample with high degre of confidence. Validation does not means that method is perfect, but validation means method has met a set of criteria to ensure that it is reliable and consistent. Tizanidine is a central alpha 2 adrenergic agonist –inhibits release of excitatory amino acid in the spinal interneurones. It may facilitate the inhibitory transmitter glycine as well. It inhibits polysyneptic reflexes reduce muscle tone and frequency of muscle spasms without reducing muscle strength.
 Following oral administration, tizanidine is essentially completely absorbed .The absolute oral bioavailability of tizanidine is approximately 40%, due to extensive first-pass hepatic metabolism. Tizanidine is extensively distributed throughout the body with a mean steady state volume of distribution of 2.4 L/kg following intravenous administration in healthy adult volunteers .tizanidine is approximately 30% bound to plasma proteins.
 Keywords: Bioanalytical method Validation, LC-MS/MS, Human Plasma, Tizanidine, HPLC.
Highlights
Bhadauria Conflict of interest statement: No conflict of interest ABSTRACT: Bioanalytical method Validation employed for quantitative determination of drug and their metabolites in biological fluids
The main purpose of method validation is to demonstrate that a specific Bioanalytical method can reliably determine the concentration of drug in study sample with high degre of confidence
Validation does not means that method is perfect, but validation means method has met a set of criteria to ensure that it is reliable and consistent
Summary
The main purpose of method validation is to demonstrate that a specific Bioanalytical method can reliably determine the concentration of drug in study sample with high degre of confidence. Bioanalytical method Validation employed for quantitative determination of drug and their metabolites in biological fluids. Following oral administration , tizanidine is essentially completely absorbed .The absolute oral bioavailability of tizanidine is approximately 40% ,due to extensive first-pass hepatic metabolism. Tizanidine is extensively distributed throughout the body with a mean steady state volume of distribution of 2.4 L/kg following intravenous administration in healthy adult volunteers .tizanidine is approximately 30% bound to plasma proteins Tizanidine has linear pharmacokinetics over a dose of 1 to 20 mg. Tizanidine has a half life of approximately 2.5 hours .approximately 95% of an administered dose is metabolized. The primary cytochrome P450 Isoenzyme involved in tizanidine metabolism is CYP1A2 Following single and multiple oral dosing the excretion of tizanidine occur through urine and feces. NAME OF MATERIAL 1 Tizanidine 2 Tizanidine d4 3 Methanol 4 Acetonitrile 5 Milli-Q-water 6 Acetic acid 7 Ortho phosphoric acid 8 Formic acid 9 Ammonium formate Ammonium acetate Tert butyl methyl ether
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