Bioalcohol production with M. aeruginosa as a novel resource

Abstract

Useful bioenergy resource without any competition with foodstuff, and if it bears fermentable higher carbohydrates, it will be novel resources for bioalcohol production. M. aeruginosa which blooms in fresh water lake and river at summer season with producing hazard toxin to animals. But it bears higher carbohydrates(56.4%) such as sucrose, cellobiose, rhamnose, galactose, xylose and glucose and also its generation time is shorter than B.braunii which is a good resource for bioenergy. And new medium for M. aeruginosa was developed with added urea 0.2g/L, increased Fe <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+2</sup> , and decreased Ca <sup xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">+2</sup> ion compared with BG11 medium. After culture with 25L and 240 litter race-way reactor, M. aeruginosa was precipitated with combination of Al <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">2</sub> SO <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">4</sub> and FeCl <sub xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink">3</sub> , precipitates were hydrolyzed with c-H2SO4, followed autoclaved, sonication and neutralization, finally the extract was used for substrate of alcohol fermentation, 120mg/ml of sugar in BG11 medium, and 140 mg/ml in modified medium were produced. Fermentation was performed with 200 ml extract in 500ml flask at 30°C for 30 hours, with 3 kinds of strain, Saccharomyces cerevisiae(Sc), Pichia stipitis(Ps), Zymomonas mobilis(Zm), and mixed these strains(Sc+Ps+Zm). Pichia stipitis(0.7%) is more suitable for producing bioalcohol for M. aeruginosa extract than other strains of Saccharomyces cerevisiae(0.45%) and Zymomonas mobilis(0.61%), while co-cultured strains showed higest product by 1.75%. M. aeruginosa will be a novel resource for bioalcohol fermentation.