Abstract
BackgroundIn folklore, some people take the decoction of Calyx seu Fructus Physalis (CSFP) for lowering blood lipids. The present study is designed to evaluate the lipid-lowering activities of CSFP, and search for its pharmacodynamical material.MethodsCSFP was extracted by water and 75% ethanol, respectively. The extracts of CSFP for reducing serum lipid levels were evaluated on mouse model of hyperlipidemia. The optimized extract was subjected to the bioactivity-guided fractionation in which the liquid-liquid extraction, collumn chromatography, the in vivo and in vitro models of hyperlipidemia were utilized. The structure of active component was determined by 13 C-NMR and 1H-NMR.ResultsThe 75% ethanol extract of CSFP decreased the serum total cholesterol (TC) and triglyceride (TG) levels in mouse model of hyperlipidemia. Followed a separation process for the 75% ethanol extract of CSFP, the fraction B was proved to be an active fraction for lowering lipid in vivo and in vitro experiments, which could significantly decrease the serum TC and TG levels in mouse model of hyperlipidemia, and remarkably decrease the increase of TG in primary mouse hepatocytes induced by high glucose and the increase of TG in HepG2 cells induced by oleic acid. The fraction B2, isolated from B on bioactivity-guided fractionation, could significantly decrease TG level in HepG2 cells. One compound with the highest content in B2 was isolated and determined as luteolin-7-O-beta-D-glucopyranoside by NMR spectra. It could significantly reduce the TG level in HepG2 cells, and inhibited the accumulation of lipids by oil red O stain.ConclusionOur results demonstrated that the 75% ethanol extract of CSFP could improve in vitro and in vivo lipid accumulation. Luteolin-7-O-beta-D-glucopyranoside might be a leading pharmacodynamical material of CSFP for lowering lipids.
Highlights
In folklore, some people take the decoction of Calyx seu Fructus Physalis (CSFP) for lowering blood lipids
Lipid-lowering effects of the extracts of CSFP on mouse model of acute hyperlipidemia As shown in Figure 1, the increases in serum total cholesterol (TC) and TG levels induced in hyperlipidemic mice were prevented by prior administration of total 75% ethanol extract (TE), compared with the model control group
Lipid-lowering effects of fractions of CSFP on primary mouse hepatocytes and HepG2 cells The cytotoxic activities of TE, E, B and W were detected by MTT
Summary
Some people take the decoction of Calyx seu Fructus Physalis (CSFP) for lowering blood lipids. The present study is designed to evaluate the lipid-lowering activities of CSFP, and search for its pharmacodynamical material. Some evidence indicates that lipid-lowering treatment can reduce progression of coronary atherosclerosis [2]. Many medicinal plants have been found to be useful to successfully manage hyperlipidemia [3,4]. The decoction of CSFP is believed to be useful as an antihyperlipidemic preparation. A survey of the literature revealed no hypolipidemic studies of this plant. The objectives of this study were to i) prepare and screen the active hypolipidemic extracts using animal models of hyperlipidemia, ii) isolate and demonstrate the active fraction(s) on bioactivity-guided separation, iii) identify and elucidate the underlying pharmacodynamical material
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