Abstract
BACKGROUND: Free radicals (oxidants) can cause skin irritation/damage which can be a manifestation of minor skin infections. Not only that, one of the complications of the disease that can arise is diabetes mellitus (DM) with diabetic foot ulcers (LKD). LKD is very susceptible to exposure to microorganisms and develops into diabetic foot infection (DFI). DFI is associated with the presence of biofilms in wounds especially those caused by Staphylococcus aureus infection. Bajakah tampala (Spatholobus littoralis hassk) is one of the native plants of Indonesia which has been known to have antibacterial activity, while its antibiofilm activity has not been studied. Evaluation of antibiofilms from the tampala bajakah plant can be of good novelty value, as well as support success in the treatment of infectious. AIM: This study aims to determine the effectiveness of the ethanol extract of the Bajakah tampala plant from East Kalimantan in inhibiting and eradicating the formation of S. aureus biofilms. MATERIALS AND METHODS: The planktonic and biofilm inhibition tests were carried out using the microtiter broth method. Antibiofilm activity of Bajakah tampala ethanol extract against S. aureus was analyzed by calculating the minimum biofilm inhibitor concentration (MBIC50) and the biofilm eradication activity calculating the minimum biofilm eradication concentration (MBEC50). In addition, we also carried out additional verification tests using the DPPH method by calculating the inhibitory concentration (IC50) parameter value. RESULTS: The results showed that the 1% bajakah tampala extract gave mid-phase antibiofilm activity of 80.23% w/v ± 0.01, maturation phase of 77.23% w/v ± 0.01 and eradication with a large inhibition of 75.56% w/v ± 0.01. In the DPPH test, the IC50 value was 5.9 ppm with a very strong category. CONCLUSION: Thus, it can be concluded that the ethanolic extract of the Bajakah tampala plant has a high potential to be developed as a candidate for new antibiofilm drugs against S. aureus biofilms.
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