Abstract

The phylum Actinobacteria recognized as the most promising producers of many bioactive compounds. Among actinobacteria, the genus Streptomyces are widely known as prolific producers of many antibiotics, for example Streptomycin. The present study investigates potential bioactivity of actinobacteria BTA 1-131 isolated from an Indonesian marine sponge Melophlus sarassinorum. Molecular identification using the 16S rRNA gene sequencing showed that the isolate is similar to Streptomyces kunmingensis (98.4%). The isolate was cultivated on to three different fermentation media (ISP2, YS, and SYP) to select the best media for bioactive compounds production. The compounds were extracted from both solid and liquid cultures using methanol as a solvent. The crude methanol extracts were tested for antibacterial and anticancer activity. The bioactivity screening indicates active potential of the extracts against bacterial pathogen Staphylococcus aureus ATCC 13420, invasive breast cancer MDA-MB-231 and human colorectal adenocarcinoma cells CaCo-2. The extracts profiling using LC-MS/MS method indicates probable influence among different fermentation media to selectively induce production of target bioactive compounds. Although further compounds dereplication and fractionation are important for specific identification, the present study demonstrates the potential of Streptomyces BTA 1-131 as producer of tetracycline and/or staurosporine. In addition to that, further genome-mining approach and analysis using a whole genome sequencing data also important to comprehensively explore the bioactive potential of the actinobacteria BTA 1-131.

Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call