Abstract
This experiment was aimed to determine the bioactivity of ethanolic extract of propolis (EEP) against the changes in the quantity of CD4 + CD25 + and B220 + lymphocytes and determine the optimal dose of EEP to increase the number of CD4 + CD25 + and B220 + cells in Balb/c mice. Balb/c mice were divided into four treatment groups: control treatment, treatments of EEP at a dose of 50 mg.kg -1 , 100 mg.kg -1 , and 200 mg.kg -1 body weight of mice. All mice were dissected after two weeks post treatment. Profiles of lymphocytes from the spleen expressing CD4 + CD25 + and B220 + cells were analyzed by flowcytometry using CellQuest software. Data was analyzed by Kruskall Wallis and Mann Whitney statistical test with P<0.05 using SPSS 16.0 for Windows. The results showed that the treatment of dose of 50 mg.kg -1 of EEP can increase relative number of CD4 + CD25 + cells significantly, but those cells decrease significantly when we apply the dose of 100 and 200 mg.kg -1 . The relative number of B220 + cells increase in the dose of 50 and 200 mg.kg -1 and decrease in the dose of 100 mg.kg -1 compared to the control. This experiment suggest that EEP has bioactivity to modulate the quantity of CD4 + CD25 + and B220 + in dependent manner. Keywords: B220 + , CD4 + CD25 + , lymphocyte, propolis
Highlights
Propolis is a natural product from a mixture of plant derived products, which modified and used by bees for various needs
This study was conducted to determine the bioactivity of ethanolic extract of propolis (EEP) on production of CD4+CD25+T cells and B220+ B cells in the spleen organ of Balb/c mice
Changes in the relative number percentage of CD4+CD25+ T cells to the lymphocyte cells population due to the effect of EEP between treatment groups generally show a decline pattern based on the results of flowcytometry analysis (Figure 1)
Summary
Propolis is a natural product from a mixture of plant derived products, which modified and used by bees for various needs. Immunomodulator is one mechanisms that needed in the body's immune system. Immunomodulatory activity is a form of biological or pharmacological effects on various factors in the immune response. The purpose of immunomodulatory itself is to modulate the homeostasis of immune system. Immunomodulatory activity used to treat and prevent various diseases originating from any occurred imbalance. The activities of immunomodulators are immunosuppressants and immunostimulant [4]. Immunomodulatory effects of propolis was known better compared to other immunomodulatory. The effect of propolis on CD4 T cell differentiation process has not known yet [6]. CD4 T cell activation is known to be influential in the process of B cell proliferation [7]. This study was conducted to determine the bioactivity of EEP on production of CD4+CD25+T cells and B220+ B cells in the spleen organ of Balb/c mice
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