Abstract
Scaffolds of polycaprolactone-30% hydroxyapatite (PCL-30% HA) were fabricated using melt stretching and multilayer deposition (MSMD), and the in vitro response of osteoblasts to the scaffolds was assessed. In group A, the scaffolds were immersed in 10 µg/mL bone morphogenetic protein-2 (BMP-2) solution prior to being seeded with osteoblasts, and they were cultured in the medium without BMP-2. In group B, the cell-scaffold constructs without BMP-2 were cultured in medium containing 10 µg/mL BMP-2. The results showed greater cell proliferation in group A. The upregulation of runt-related transcription factor 2 and osteocalcin genes correlated with the release of BMP-2 from the scaffolds. The PCL-30% HA MSMD scaffolds appear to be suitable for use as osteoconductive frameworks and BMP-2 carriers.
Highlights
Dose bone morphogenetic protein-2 (BMP-2): An In Vitro Study.Severe alveolar bone loss and jawbone atrophy are common problems associated with denture and implant construction in elderly patients
The PCL-30% HA scaffolds were fabricated using the melt stretching and multilayer deposition (MSMD) technique according to the following protocol [29]: the PCL pellets and the HA particles were homogeneously mixed in the ratio of PCL:HA = 70:30 by weight in a melt extrusion machine at 140 ◦ C
This study demonstrated the efficacy of low doses of BMP-2 for enhancing growth and differentiation of bone forming cells in 2-D and 3-D culture models
Summary
Severe alveolar bone loss and jawbone atrophy are common problems associated with denture and implant construction in elderly patients. The graft particles are thought to act as osteoconductive frameworks that support new bone regeneration in the defect areas. The primary problem is that the graft particles cannot maintain their volumes and shapes by themselves and so additional rigid covering membranes or mesh trays are needed. In these cases, using scaffold-formed bone substitutes could overcome this problem and increase the success rate of these treatments [1,2,3,4,5,6]
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