Bioactivity assay of Arundina graminifolia (D.Don) Hochr. extracts from diverse plant parts in Thailand: An assay-based investigation

Abstract

This research involved surveying and collecting 16 samples of Arundina graminifolia from 8 different subpopulation areas. The main objectives were to analyze the preliminary phytochemical fingerprints and biological activity of the crude extracts obtained from various parts of the plant (roots, rhizomes, stems, and leaves). The findings indicated that rhizome extracts generally yielded a higher percentage of crude extract compared to the dry plant weight in comparison to other plant parts. Moreover, the Thin layer chromatography (TLC) fingerprint data revealed distinct compositions for each part of the plant, with rhizomes exhibiting more prevalent bands of compounds than other parts. Regarding antioxidant activity, the root and rhizome crude extracts from various populations displayed stronger inhibitory activity against DPPH, ABTS, and FRAP methods compared to stems and leaves. High total phenolic content was also found to effectively inhibit free radicals. The lipoxygenase inhibition test, which serves as an in vitro anti-inflammatory activity evaluation, demonstrated that most root extracts exhibited higher lipoxygenase inhibitory activity than the rhizome extracts at a concentration of 0.1 mg/mL. The antibacterial activity demonstrated that rhizome extracts from different regions exerted lower MIC and MBC values against all tested bacteria compared to the root extracts. The findings offer valuable information for selecting specific populations of A. graminifolia as potential sources of bioactive compounds for future nutraceutical applications.