Bioactive Volatile Content of the Stem and Root ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis

Ibrahim Demirtas,Ayse Sahin

doi:10.1155/2013/125286

Abstract

Several species ofCentaurea, biennial (or more rarely perennial) plants which belong to the Asteraceae family, possess medicinal properties and are currently used in phytotherapy. In the present study, antiproliferative activity of hexane extract from stems and roots ofCentaurea carduiformisDC. subsp.carduiformisvar.carduiformis(CCS and CCR) on Vero cells (African green monkey kidney), C6 cells (Rat Brain tumor cells), and HeLa cells (human uterus carcinoma) was investigatedin vitro. Antiproliferative effect of the extract was tested at 500 μg/mL and 1000 μg/mL using BrDu Cell Proliferation ELISA. The hexane extract was significantly inhibited proliferation of Vero, HeLa, and C6 cancer cell lines with absorbance values. The extract of CCS and CCR showed the highest activity against the Vero, HeLa, and C6 cell lines at 500 μg/mL and 1000 μg/mL.

Highlights

E study of lipids has drawn increasing attention in many elds of research and application due to the existence and functioning of living organisms
As a continuation of our research on the volatile components of some Centaurea species [15,16,17,18,19] in the present study, we report the fatty acids of the stem and root of endemic Centaurea carduiformis (CC) plant distributed in the black sea area
In the present study to determine whether the reduction in cell number mediated by UFAs and SFAs was a result of a cytotoxic effect from fatty acids, we monitored the viability of cells during the course of incubation

Summary

Introduction

E study of lipids has drawn increasing attention in many elds of research and application due to the existence and functioning of living organisms. Erefore, with the increasing acceptance of traditional medicine as an alternative form of health care, the screening of plants for active compounds has become very important. As a continuation of our research on the volatile components of some Centaurea species [15,16,17,18,19] in the present study, we report the fatty acids of the stem and root of endemic Centaurea carduiformis (CC) plant distributed in the black sea area. To the best of our knowledge, no analyses have been previously reported on antiproliferative activity of Centaurea carduiformis DC. Fatty acids of CC on roots (CCR) and stems (CCS) were obtained and tested for antiproliferative activity for Vero, C6, and HeLa cells

Methods

Results

Conclusion