Abstract
Objective: Antibiotic resistance of pathogens has become a serious problem all over the world. Therefore, focusing for novel antibiotics is an important endeavor which is very much needed. Around 50 morphologically different Actinobacteria isolated from mangrove habitats of Krishna district, Andhra Pradesh, India were screened for antimicrobial activity. Methods: Among 50 isolates, one strain Nocardiopsis synnemataformans VLS-10 was identified based on morphological, physiological, biochemical, and molecular approaches. The effect of environmental parameters such as incubation period, pH, temperature and salt concentration, and effect of various nutrients such as carbon and nitrogen sources and minerals on the bioactive metabolite production by N. synnemataformans VLS-10 was evaluated by employing agar well diffusion assay. Results: Among the 50 isolates, one strain designated as VLS-10 was efficient to produce potential secondary metabolites. It was identified as N. synnemataformans based on polyphasic taxonomy. The present work is mainly aimed to study process optimization parameters to get high yield of bioactive compounds. ISP-2 medium supplemented with sodium chloride @ 9% maintained at pH 7.0 supported maximum yield of secondary metabolites by the strain when incubated at 35°C for 9 days. Conclusion: Secondary metabolites possessed broad-spectrum activity against human pathogenic bacteria and fungi. Hence, strain N. synnemataformansVLS-10 becomes a significant source for antimicrobial compounds.
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