Abstract
Bone defect repair in osteoporosis remains a major clinical problem due to the persistent abnormal inflammatory response and imbalance of bone homeostasis. Manganese (Mn) ions have been proven to have dual effects of promoting immunomodulation and osteogenesis, showing great potentials for bone tissue regeneration in osteoporotic bone defects. In the present study, a novel Mn2+-releasing coating on titanium (Ti) substrates was prepared via biomimetic mussel-inspired chemical modification. Notably, the combination of polydopamine (PDA) and Mn2+ endowed Ti@PDA+Mn with diverse bioactivities. In addition to excellent biocompatibility and osteogenic capability, Ti@PDA+Mn exhibited the ability to regulate intracellular reactive oxygen species (ROS) and induce macrophage to polarize towards M2 phenotype by the secretion of anti-inflammatory cytokines such as IL-10 and osteogenic-related cytokines such as TGF-β, establishing an osteoimmune microenvironment that facilitates bone tissue repair, establishing an osteoimmune microenvironment that facilitates bone tissue repair. In the ovariectomized osteoporosis rat model, Ti@PDA+Mn exhibited enhanced osseointegration in femoral bone defects demonstrated by micro-CT, histopathology, and immunohistochemistry at 2 and 6 weeks after surgery. Finally, the potential signaling pathway of Mn2+ regulating osteogenesis has been further studied. Transcriptome sequencing revealed the significant regulatory functions of Mn2+, which upregulated the HIF-1 signaling pathway to enhance bone regeneration. The mechanism of Mn-enhanced osteogenesis developed in this study could provide theoretical and practical insights for enhancing implant-to-bone osseointegration in osteoporotic bone defects.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Similar Papers
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.