Bioactive Glass-Green and Red Propolis Association: Antimicrobial Activity Against Oral Pathogen Bacteria

Abstract

Bioactive glasses were studied as potential drug delivery candidates for the incorporation and release of natural products. In light of these studies, the antimicrobial activity of a new formulation containing propolis was evaluated. Susceptibility of oral pathogens microorganisms were evaluated using the CLSI standard MIC, MBC and agar diffusion method with Brazilian green (BGP) and red (BRP) propolis extracts isolated and associated with bioglass (BGGP and BGRP). Isolated Bioglass (BG), Tetracycline (TT) and Ethanol (ET) were the controls. Results data were expressed as M ± SD. Significant differences between microorganisms treated groups antimicrobial assays were analyzed by Student’s-test (significance at) using the Origin 6.0 program. All tested bacteria were susceptible to bioglassincorporated green and red propolis. The new formulation was effective for inhibiting the in vitro microorganisms growth after 24 and 48 hours at 37°C in an atmosphere specific to each microorganism. Red propolis being more active than the green one. Propolis incorporated into bioglass did not lose its antimicrobial properties. The positive results suggest that propolis in this sustained release formulation should be further tested as an alternative therapy against infectious agents of the oral cavity.