Abstract
As a Mongolian native medicine and Ewenki folk medicinal plant, Gentianella acuta has been widely used for the treatment of diarrhea, hepatitis, arrhythmia, and coronary heart disease. In the course of investigating efficacy compounds to treat diarrhea using a mouse isolated intestine tissue model, we found 70% EtOH extract of G. acuta whole plants had an inhibitory effect on intestine contraction tension. Here, nineteen constituents, including five new compounds, named as gentiiridosides A (1), B (2), gentilignanoside A (3), (1R)-2,2,3-trimethyl-4-hydroxymethylcyclopent-3-ene-1-methyl-O-β-d-glucopyranoside (4), and (3Z)-3-hexene-1,5-diol 1-O-α-l-arabinopyranosyl(1→6)-β-d-glucopyranoside (5) were obtained from it. The structures of them were elucidated by chemical and spectroscopic methods. Furthermore, the inhibitory effects on motility of mouse isolated intestine tissue of the above mentioned compounds and other thirteen iridoid- and secoiridoid-type monoterpenes (7–10, 13–16, 18, 19, 21, 22, and 25) previously obtained in the plant were analyzed. As results, new compound 5, some secoiridoid-type monoterpenes 7, 10, 12–14, 16, and 17, as well as 7-O-9′-type lignans 31 and 32 displayed significant inhibitory effect on contraction tension at 40 μM.
Highlights
Gentianella acuta (Michx.) Hulten belongs to the family Gentianaceae, distributed mainly in the north of China, Mongolia plateau, Siberia, and Far East areas of Russia [1]
They have on motility isolation isolation and and structure structure elucidation elucidation of of them, them, along along with with evaluations evaluations of of their their inhibitory inhibitory effects effects on on mouse isolated intestine tissue, too? In this paper, we describe the isolation and structure elucidation intestine contraction tension
The relative configuration of 1 was determined by a nuclear Overhauser effect spectroscopy (NOESY) experiment, and NOE correlations were observed between H-1 and H-8; H-5 and H-7; H3 -10 and H-7, H-9
Summary
Gentianella acuta (Michx.) Hulten belongs to the family Gentianaceae, distributed mainly in the north of China, Mongolia plateau, Siberia, and Far East areas of Russia [1]. In the course of investigating efficacy compounds to treat diarrhea using a mouse isolated intestine tissue model, we found that a 70% EtOH extract of G. acuta whole plants had an inhibitory effect on intestine contraction tension. Eighteen xanthones had been obtained and their inhibitory effects on the model were assayed. Some xanthones were found to have a significant reducing effect on intestine contraction tension [4]. 22 of of 13 we obtained other nineteen constituents, including five new compounds (1–5) (Figure 1) and fourteen inhibitory effect on motility of mouse isolated intestine tissue, too?. We describe the isolation and structure elucidation intestine contraction tension. Of them, along with evaluations of their inhibitory effects on intestine contraction tension. Figure obtainedfrom fromthe thewhole wholeplant plantof acuta
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