Bioactive compounds sourced from Terminalia spp. in bacterial malodour prevention: an effective alternative to chemical additives.

Abstract

Recently, our group reported that extracts prepared from the Australian native plant Terminalia ferdinandiana Exell. are potent inhibitors of the growth malodorous bacteria with similar efficacy to triclosan and through these results, we highlighted a potential biological alternative to the current chemical additives. Other members of the genus Terminalia are also well documented for their antibacterial potential and tannin contents and thus were investigated as potential deodorant additives. Solvent extractions prepared from of selected Indian, Australian and South African Terminalia spp. were screened by disc diffusion and liquid dilution assays against C.jeikeium, S.epidermidis, P.acnes and B.linens. The antibacterial activity was quantified by liquid dilution MIC assays. The extracts were screened for toxicity using Atremia franciscana nauplii and HDF cell viability bioassays. High-resolution time-of-flight (TOF) LC-MS and GC-MS headspace fingerprint analysis was used to detect tannin, flavonoid and terpenoid components in the extracts. Bacterial growth inhibition was observed in all Terminalia extracts with the methanolic T.chebula, T.carpenteriae and T.sericea extracts the most promising bacterial growth inhibitors, yielding MIC values as low as 200µgmL-1 . Toxicity analyses of the extracts were favourable, and we determined that the methanolic T.chebula, T.carpenteriae and T.sericea extracts were all non-toxic. Using previously detected T.ferdinandiana antimicrobials as benchmarks, LC-MS and GC-MS fingerprint analyses revealed similar compounds in the methanolic T.chebula, T.carpenteriae and T.sericea extracts. Through these results, we propose that Terminalia spp. extracts may be useful deodorant additives to inhibit the growth of axillary and plantar malodorous bacteria, offering a biological alternative to their chemically synthesized counterparts.