Abstract
Algae Kappaphycus alvarezii is one of the leading commodities from fisheries and marine sectors in Indonesia. This algae species has many benefits in the fields of marine biology, chemistry, pharmacy, and pharmacology, as well as an excellent nutritional content for health. It is cultivated in the coastal waters of Minahasan Peninsula, especially in Buku Village, Belang District, Southeast Minahasa Regency. This study was carried out to analyze bioactive compound in the algae. Samples of 2-weeks old were taken directly from the cultivation area. Screening test was done qualitatively while an advanced test was done using LC-HRMS method. The results showed 4 bioactive compounds detected in the sample, they are alkaloids, tannins/phenolics, saponins, and flavonoids. From the advanced test, it was found 10 major compounds in the sample, i.e. (2-ethylhexyl) phthalate, phthalic acid, D-(+)-proline, maraniol, stigmatellin Y, Pentyl isopentyl phthalate, methyl thiofentanyl, phenylethanolamine, acetyl hexamethyl tetralin, and fucoxanthin.Indonesian title: Senyawa bioaktif pada alga Kappaphycus alvarezii dari perairan Belang, Kabupaten Minahasa Tenggara
Highlights
Sumber daya hayati laut perlu dimanfaatkan secara optimal
The results showed 4 bioactive compounds detected in the sample, they are alkaloids, tannins/phenolics, saponins, and flavonoids
Ditemukan juga 10 senyawa utama, yaitu (2-ethylhexyl) phthalate, phthalic acid, D-(+)-proline, maraniol/methyl coumarin, stigmatellin Y, pentyl isopentyl phthalate, methyl thiofentanyl, phenylethanolamine, acetyl hexamethyl tetralin/ tonalide, dan fucoxanthin
Summary
Pengambilan sampel alga K. alvarezii dilakukan di perairan pesisir Minahasa Tenggara, Desa Buku Kecamatan Belang, Provinsi Sulawesi Utara (Gambar 1). Sampel berumur 2 minggu, diambil secara langsung dari lahan budidaya rumput laut. Identifikasi morfologi sampel alga K. alvarezii dilakukan di Laboratorium Biomolekuler dan Farmasitika Laut, Fakultas Perikanan dan Ilmu Kelautan, Universitas Sam Ratulangi (Unsrat) Manado, menggunakan panduan Trono (1997). Untuk mengetahui senyawa bioaktif di dalam sampel, 2 macam analisis laboratorium dilakukan, yaitu skrining fitokimia, yang dilakukan di Lab. FMIPA Unsrat, dan pengujian lanjut, yang dilakukan di Lab. Sentral Ilmu Hayati, Universitas Brawijaya, Malang, menggunakan Liquid ChromatographyHigh Resolution Mass Spectrometry (LC-HRMS). Senyawa bioaktif yang terkandung pada sampel diuji secara kualitatif berdasarkan perubahan warna atau endapan yang terbentuk sebagai respon terhadap reagen yang diberikan
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