Bioactive Compounds and Antioxidant Activity in Greek-style Yoghurt Infused with Shield Aralia Leaves

Abstract

The objective of this study was to determine bioactive compounds, nutrition, antioxidant activity, total phenolic content (TPC) and total flavonoid content (TFC) in shield aralia yoghurt. Shield aralia leaves (SAL) have traditionally been used as herbal plant. Fortification of shield aralia in yoghurt increased the health-benefits of yoghurt by the incorporated phytochemical constituent of herb such as polyphenol and flavonoid groups which can be used to treat various disease including atherosclerosis, obesity, diabetes mellitus, and cardiovascular disease. The Greek-style yoghurt with addition SAL resulted on new bioactive compounds belong to α-linoleic acid (ALA) including -hydroperoxy-octadecadienoic acid (9-HpODE), (15Z)-9,12,13-Trihydroxy-15-octadeconoic acid, 13(s)-hydroperoxylinolenic acid (13(S)-HPOTrE), and (E)-6-hydroxyoctadec-4-eonoic acid or NP-011548. The proximate test results revealed that shield aralia yoghurt has high-protein content (15.46%), low-fat and carbohydrate content (0.16% and 0.88%, respectively). The antioxidant activity based on 2, 2-duphenylpicrylhydrazyl (DPPH) assay of shield aralia yoghurt was 68.66%, while TPC of SAL yogurt reached 62.61 mg GAE/g and the TFC was 201.05 mg QE/g. These results suggested that under fermentation by lactic-acid bacteria (LAB) increased the beneficial values of shield aralia leaves compared with shield aralia raw extract. Shield aralia yoghurt could be as promising supplementation for wellness care and also could be as exogenous antioxidant to increase body defense.