Abstract
The use of liquid organic amendments (biols) is a common practice for farmers due to the multiple benefits in the management and production of their crops, including the control of pests and diseases. The present study analyzes the chemical composition of the pure compound C1 and fractions F2 and F3 of local biols produced in two provinces of Ecuador and their antifungal activity against Moniliophthora roreri (Cif & Par). This work incorporates the use of Gas Chromatography coupled to Mass Spectrometry (GC-MS), Nuclear Magnetic Resonance (NMR) and in vitro inhibition assays for sample analysis. C1 was identified as Mannitol. The percentage of inhibition against M. roreri in F2 and F3 was 44.37% and 8.34%, respectively; and, for C1, 28.63%. The values of the median lethal dose (LD50) obtained corroborate that the F2 fraction was the one with the highest controlling activity against the pathogen. The 1,2-benzenedicarboxylic acid diisooctyl ester compound was the main compound in F2 (30.88%) and the Pentadecanoic acid, 14-methyl-, methyl ester in F3. Finally, all compounds obtained from the biol fractions were identified and it was determined that the fermentative process was suitable for producing bioactive compounds of interest to inhibit the growth of Moniliophthora roreri.
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