Bioactive Compound Contents and Biological Activities of the Algerian Medicinal Plant Rhus Pentaphylla (Jacq.) Desf. (Anacardiaceae)

Abstract

Rhus pentaphylla (Jacq.) Desf. is an Algerian food and medicinal specie used to treat diarrhea and abdominal colic and is employed as a digestive [1,2,3], yet there is little information available concerning its bioactive composition. The aim of this study was to quantify bioactive compounds and to investigate antioxidant and acetylcholinesterase inhibitory activities of extracts obtained from leaves of R. pentaphylla in order to prove its possible use as potential natural source for human health. The bioactive compounds were quantified using colorimetric methods. The phytochemical analysis was carried out using chromatographic techniques. The antioxidant activity was assayed using several test systems, namely total antioxidant capacity, DPPH, ABTS and OH radical scavenging activity, a ferric reducing power assay, and a ferrous ion chelating capacity and β-carotene bleaching assay. The acetylcholinesterase inhibitory activity was determined using bioautographic and spectrophotometric methods. The results showed that extracts of R. pentaphylla are an excellent source of phenolic compounds, especially regarding its high flavonoid and hydrolysable tannin contents (36.26 ± 1.77 mg CE/g DE and 898.64 ± 26.56 mg TAE/g DE, respectively), promoting high antioxidant activity. The phytochemical analysis led to the identification of several phenolic compounds that were dominated by flavonoids and phenolic acids. The extracts showed potential antioxidant activity in the different tests. This could be attributed to their polyphenol, tannin, and flavonoid contents. The extracts of R. pentaphylla also exhibited anti-acetylcholinesterase activity. Owing to its phenolic profile and biological activities, R. pentaphylla could be considered as potential functional ingredient for pharmaceutical applications.