Bioaccumulation of Cu, Fe, Mn and Zn in native Brachystegia longifolia naturally growing in a copper mining environment of Mufulira, Zambia

Abstract

Heavy metal contamination in the soil and the subsequent accumulation in Brachystegia longifolia were investigated as a function of the wind direction and distance from a copper mine in Mufulira, Zambia. Soil and leaves of B. longifolia were collected along transects up to 12km downwind and 19km upwind. The total concentration of trace elements in the soil and leaves was determined through pXRF. Plant-available Cu, Fe, Mn, and Zn were extracted in a Mehlich III solution and analyzed using ICP-AES. The degree of soil contamination illustrates that Cu and Fe from the copper mine strongly pollute Mufulira and the surrounding forests. Bioavailable Cu, Fe, Mn, and Zn reduced with increasing distance from the mine. An average of 296mg/kg Cu, 2337mg/kg Fe, 1101mg/kg Mn, and 109mg/kg Zn were recorded in leaves at the most polluted site. Similarly, 55.21mg/kg Cu, 516.4mg/kg Fe, 3196mg/kg Mn, and 154mg/kg Zn were recorded at an unpolluted site 19km upwind. The concentration of Cu and Fe reduced significantly with increasing distance, while Mn and Zn increased significantly. It was further established that B. longifolia leaves accumulated Mn (× 38) and Zn (× 15) more than their respective total concentration in the soil. The concentrations of Cu and Fe found in leaves near the mine, as well as the Mn concentration in leaves across the study sites, could be stressful for B. longifolia tree growth.