Bioaccumulation of β-methylamino-L-alanine (BMAA) by mussels exposed to the cyanobacteria Microcystis aeruginosa

Abstract

Cyanobacterial blooms are increasingly common in aquatic environments, raising concerns about the health impacts associated with the toxins they produce. One of these toxins is β-methylamino-L-alanine (BMAA), a neurotoxin linked to neurodegenerative diseases. Monitoring BMAA levels in the environment is challenging due to trace concentrations and complex matrices, and new approaches are needed for assessing exposure risk. In this laboratory study, Australian freshwater mussels, Velesunio ambiguus, were exposed to a BMAA-producing cyanobacterium, Microcystis aeruginosa, to assess its accumulation of the toxin over time. A sample preparation and analysis method was developed to allow accurate quantification of BMAA in the mussels at concentrations as low as 0.4 ng/g. Mussels exposed to M. aeruginosa accumulated BMAA, with concentrations increasing over the exposure period. Rapid depuration occurred after exposure to the cyanobacterium ended, with concentrations of BMAA quickly returning to pre-exposure levels. These results demonstrate the potential for mussels to be used as bioindicators in the field for monitoring BMAA levels over time, where rapid depuration is unlikely.