Bioaccumulation and toxicity of perfluorooctanoic acid and perfluorooctane sulfonate in marine algae Chlorella sp.

Abstract

The ocean is an important sink for perfluorinated alkyl acids (PFAAs), but the toxic mechanisms of PFAAs to marine organisms have not been clearly studied. In this study, the growth rate, photosynthetic activity, oxidative stress and bioaccumulation were investigated using marine algae Chlorella sp. after the exposure of perfluorooctanoic acid (PFOA) and perfluorooctane sulfonate acid (PFOS). The results showed that PFOA of <40 mg/L and PFOS of <20 mg/L stimulated algal reproduction, and high doses inhibited the algal growth. The absorbed PFOA and PFOS by algal cells damaged cell membrane and caused metabolic disorder. The photosynthesis activity was inhibited, which was revealed by the significantly reduced maximal quantum yield (Fv/Fm), relative electron transfer rate (rETR) and carbohydrate synthesis. However, the chlorophyll a content increased along with the up-regulation of its encoding genes (psbB and chlB), probably due to an overcompensation effect. The increase of ROS and antioxidant substances (SOD, CAT and GSH) indicated that PFOA and PFOS caused oxidative stress. The BCF of marine algae Chlorella sp. to PFOA and PFOS was calculated to be between 82 and 200, confirming the bioaccumulation of PFOA and PFOS in marine algae. In summary, PFOA and PFOS can accumulate in Chlorella sp. cells, disrupt photosynthesis, trigger oxidative stress and inhibit algal growth. PFOS shows higher toxicity and bioaccumulation than PFOA. The information is important to evaluate the environmental risks of PFAAs.