Abstract
Hydrolyzed collagen consists of peptides, which exert important biological functions in different body systems. This study aimed at testing the biological effects of a low molecular weight collagen (LMWC), namely Shore Magic® Collagen (SMC), in a series of in vitro assays and three different in vitro barrier models with translational significance. We also compared SMC's biological activities with its trypsinized form (TSMC). SMC enhanced migration in both epithelial and endothelial cells; and increased the adhesion of epithelial cells, but surprisingly not of endothelial cells. It also diminished the tightness in the gut and blood-brain barriers in vitro while TSMC did not. SMC induced both neurogenesis and BJ epithelial cell proliferation of cells growing below the in vitro barriers. In conclusion, the intact form of SMC shows enhanced bioavailability and efficiency compared with TSMC.
Highlights
Proteins comprise almost 15% of the human body
Caco-2 cells were cultured in high glucose Dulbecco’s Modified Eagle Medium; SH-SY5Y cells were cultured in a Roswell Park Memorial Institute (RPMI) medium; human umbilical vein endothelial cells (HUVEC) cells were cultured in Endothelial Cell Growth Medium; and BJ and HFF cells were cultured in BIOAMFÔ-2 Amniotic Fluid & Chorionic Villus Cell Culture Medium
We managed to demonstrate important in vitro mechanisms of action caused by the hydrolyzed collagen Shore MagicÒ Collagen (SMC), which has already proved its clinical effects in the clinic:
Summary
Proteins comprise almost 15% of the human body. Collagen is the major protein of the extracellular matrix (ECM) and is the most abundant in mammals, representing 70%e80% of skin proteins (Brett, 2008). Types I, II, and III are the main types of collagen found in connective tissue and constitute 90% of all collagen in the body (Brett, 2008). The development of more collagen supplements brought to the market hydrolyzed collagen with higher bioavailability. Hydrolysate Collagen (HC) consists of small peptides with a low molecular weight (lower than 5.000 Da.), which are produced from gelatinization and enzymatic hydrolysis of collagen derived from animal tissues (Bello and Oesser, 2006). A study using a nutritional supplement consisting of hydrolyzed collagen, hyaluronic acid, vitamins, and minerals demonstrated a reduction in skin dryness, wrinkles, and nasolabial fold depth, along with a significant increase in collagen density and skin firmness (Sibilla and Borumand, 2014)
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