Bio-remediation of crude oil contaminated soil using recombinant native microbial strain

Abstract

The manufacture, carriage, refining, and storage of crude oil are several surface and subsurface soil pollution sources. In crude oil handling operations, there is a significant number of polluted sites worldwide. This research was performed on trays with particular prominence in the biodegradation of n-alkanes to biodegrade artificially produced oil-polluted soil by microorganic strains. Two native isolates from five isolates were selected for preliminary oil degradation potential study. Previously microbial strains are isolated from crude oil contaminated soil collected from nearby oil well areas in Thiruvarur district, Tamil Nadu state, India. One promising isolate among the two microbial strains is selected for recombinant progression after completing the recombinant process. The wild and recombinant microbial strain was inoculated in the soil at ambient conditions for 42 days with different oil concentrations of 0.5%, 1%., 3%, and 5%. The total petroleum hydrocarbon (TPH) analysis has been quantitatively tracked. The biodegradation process is monitored by a gas chromatographic–mass spectrometer (GC–MS) analysis. Following 42 days of incubation, the findings showed a higher TPH biodegradation performance of the recombinant microbial strain than that of the wild microbial strain. The recombinant microbial strain showed total normal alkanes degradation significantly. This work will untie a new corridor of bioremediation of crude oil contaminated soil using recombinant microbial strain. • A detailed study on oil contamination caused by the spillage in oil drilling stations in Cauvery delta region. • Bio-remediation and microbial and molecular techniques have been discussed thoroughly. • Pseudomonas guguanensis bacteria were isolated in Indian hydrocarbon contaminated soil. • Biodegradation and evaluation of hydrocarbon in contaminated soil are experimentally studied. • Recombinant strain biodegradation potential is high compared to native microorganisms.