Abstract
This research aimed to valorize brewery spent grain (BSG) using subcritical water hydrolysis (SWH) modified by six different natural deep eutectic solvents (DES) and determining their bipotentialities. DES-induced SWH BSG hydrolysates showed the highest sugar content 46.63 ± 2.10 mg glucose, protein 228.08 ± 5.52 mg BSA, total flavonoids 22.87 ± 0.23 mg RE, phenolic compounds 8.00 ± 0.25 mg GAE from per gram dried BSG. A total of 9 different essential amino acids were identified from the BSG-hydrolysates; among them, leucine, isoleucine, phenylalanine, and histidine were the most prominent. The major phenolic compounds in the hydrolysates are gallic acid, protocatechuic acid, catechin, 4-hydroxybenzoic acid, naringin, and ferulic acid. BSG hydrolysates demonstrated antimicrobial activities and FT-IR analysis confirmed the presence of bioactive compounds. This comprehensive profiling of different bioactive compounds from BSG using DES-mediated SWH can be helpful for the efficient valorization of BSG for industrial purposes.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
