Abstract
Photobiological H 2 production is a promising method for renewable energy development. An innovative system that co-cultivating Rhodopseudomonas palustris WP3-5 and Anabaena sp. CH3 was carried out to estimate the effect of co-cultivation on H 2 production enhancement. H 2 production prolongation and enhancement were observed due to the light and metabolic compatibility of these two strains. Co-culture system served by acetate and fructose as carbon source can accumulate H 2 in 140.8 mL, almost double than the sum of individuals. Moreover, the enhancement of H 2 production was significantly affected by the mixed ratio of two strains. The mixed ratio (WP3-5:CH3) of 1:2 showed a highest H 2 production rate in 44.8 mL-H 2/L-culture/h, and both 2:1 and 1:2 exhibited a relatively high substrate conversion efficiency during the latest period of cultivation, whereas the mixed ratio of 1:1 and 3:1 only revealed a prolongation in H 2 production due to metabolic compatibility of two strains.
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