Bio-hybrid inorganic microparticles derived from CO2 for highly efficient and selective removal of antibiotics

Sang Hoon Kim,Ee Taek Hwang,Man Bock Gu

doi:10.1186/s13036-018-0113-8

Abstract

BackgroundAntibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drug-resistant bacteria, and accumulating in the food chain system. Among antibiotics, oxytetracycline (OTC) is widely used in aquaculture, and its potential risk of toxicity to human by bioaccumulation has been reported. Therefore, the effective removal of OTC is highly needed.ResultsIn this study, we report bio-hybrid inorganic microparticles (apt-mag-SiCC) for efficient capturing and facile magnet-based separation of oxytetracycline (OTC). These bio-hybrid inorganic microparticles are composed of magnetic separable silica coated calcium carbonate microparticles (mag-SiCC) derived from CO2, conjugated with oxytetracycline binding aptamers (OBA). These bio-hybrid inorganic microparticles were successfully synthesized, based on the characterization data obtained by SEM, FT-IR, EDAX, BET, and CLSM. About 6 μm sized bio-hybrid inorganic microparticles showed low non-specific adsorption to OTC and other molecules, and the selective capturing towards to the OTC in both buffer and tap water. Moreover, these bio-hybrid mineral microparticles were found to be stable, even after the repeated usages, maintaining the initial capturing efficiency.ConclusionUsing the newly synthesized bio-hybrid inorganic microparticles, we could successfully capture OTC by facile magnet-based separation. With advantages of theses bio-hybrid inorganic microparticles such as easy fabrication, low-price, and environmental friendliness, this novel material could be utilized in the drinking water treatment, in vitro medicinal diagnostics, or in vitro removal of antibiotics lining out from the blood (blood purification).

Highlights

Antibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drugresistant bacteria, and accumulating in the food chain system
In conclusion, we have shown the efficient and selective chemical capturing by using so-called bio-hybrid inorganic microparticles for the first time
These bio-hybrid inorganic microparticles were characterized by XRD, SEM, FT-IR, EDAX, BET, and confocal laser scanning microscope (CLSM)

Summary

Introduction

Antibiotics, which are the most important medication in human history, have brought global concerns due to their potential risk to human health and environment by accelerating the development of drugresistant bacteria, and accumulating in the food chain system. More than 250 antibiotics has been registered, and it is excessively used in human medicinal treatment and in the livestock industry and aquacultures [3] They can contaminate the soils, ground waters, drinking waters through the various routes [4]. It accelerates the development of drug-resistant bacteria, There are diverse methods for removing and degrading antibiotics, such as oxidation and adsorption techniques [8, 9]. Such techniques possess disadvantages including intensive energy consumption, toxicity concerns after treatment, difficulty in repetitive usages and so on [10, 11]. Specific capturing of antibiotics using affinity elements such as an antibody, Kim et al Journal of Biological Engineering (2018) 12:16 aptamer, and so on, can be one solution to overcome these drawbacks

Methods

Results

Conclusion