Bio-Electrochemical Drug Metabolism and Inhibition: Underrated Liver Microsomal Bioelectrodes

Abstract

From the initial attempts to deliver electrons to bulk microsomal solutions (1980s), and subsequent successful bioactive microsomal biofilm designs on electrodes (mid 2000s), the topic of bio-electrochemical cytochrome P450 has yielded commendable knowledge. Co-factor free, label-free, and stereoselective P450-specific metabolite production and isoform identification have immense potential for pharmaceutical drug development, metabolite profiling, and as a cost-effective complementing approach to tedious chemical synthesis, cell, and biological assays. In comparison to the progress on studying purified isolated electrocatalytic enzymes including cytochrome P450s, liver microsomal electrocatalysis is relatively a new topical area with many unknowns. Advancements and filling the knowledge gaps on the microsomal P450 electrocatalysis with nanomaterials and throughput designs hold the translational potential for practical usability by pharmaceutical industry. In this presentation, we share our contributions on various successful electro-interfacing of human liver microsomes with a range of electrode materials and nanomaterials for voltage-driven biocatalysis.