Abstract
In this paper, we investigated the bio-effect of three kinds of nano-TiO 2 on the aspects of DNA strand breaking activity, generation of 8-hydroxy-2-deoxyguanosine (8-OHdG), cytotoxicity and cell morphology. Different extents of strand scission and the formation of 8-OHdG in DNA were obtained after treated with different types of nano-TiO 2 (10 – 20 nm anatase, 50 – 60 nm anatase and 50 – 60 nm rutile). Furthermore, MTT assay and TEM detection showed the toxic effects on CHO cells with a toxic order as 10 – 20 nm anatase > 50 – 60 nm anatase > 50 – 60 nm rutile, which was consistent with that of 8-OHdG generation and DNA strand breaking activities. It was interesting that three kind of nano TiO 2 all had much higher toxic effect on tumor cells than on normal cells, and nano-TiO 2 was observed in the cytoplasm of CHO cells. These results indicated that the toxic effect of nano-TiO 2 relates to the size and type of particles, in addition, their potential application for cancer treatment was discussed.
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