Bio-control trials ofChaetomium spirale ND35 against apple canker

Abstract

A new endophytic antagonistic fungus,Chaetomium spirale ND35 fromPopulus tomentosa, was reported. The bio-control trials ofC. spirale ND35 against theValsa Canker of apple were preliminarily investigated. The results of dual culture on PDA plate showed that C.spirale ND35 was capable of strong antagonism againstValsa ceratosperma, and for inhibiting the mycelial growth ofV. ceratosperma., the crude extract of liquid culture of corn steep powder broth was more effective than that one of malt extract broth (MEB). The results of bio-control in greenhouse and field indicated that the disease incidence of apple tree treated withC. spirale ND35 was lower significantly than that treated by other methods. The re-isolation experiment suggested thatC. spirale ND35 could colonize in stems and branches of apple trees successfully, and the ND35 colonization rate of the treatment with solid wheat bran culture was higher than that of corn steep powder broth, but the field experiment result the control effect of liquid culture ofC. spirale ND35 was better than that of solid culture.