Abstract
Interestingly, antifungal protein AFP was purified from Aspergillus giganteus supernatants with modified isoelectric focusing procedure after adaptation of the secretion conditions. Subsequently, the antifungal activity as well as the mode of action against Alternaria alternata was tested in vitro. Moreover, different concentrations of AFP were applied to banana fruits for 15 days at 20°C in vivo. Obtained results illustrated that A. giganteus was able to secrete about 39.78 ± 2.39 mg AFP·l-1 Olson medium. The employed ammonium sulfate (AS 75%) precipitation procedure followed by dialysis steps yielded about 16 - 22 mg AFP·l-1 culture supernatant with general mean of 18.67 ± 1.98 mg·l-1. The lost amount of AFP during purification using AS and 3KDa cut-off dialysis membrane is about 50% thus, purification procedure must be further improved. Indeed, concluded results from MIC and hyphal extension inhibition test noticed that AFP was efficiently affected by either growth or hyphae form of A. alternata in vitro. The MIC of AFP against A. alternata was 2μg·ml-1. However,short, thick and highly septated hyphae with damaged constricted apical regions extruding from condensed mycelium aggregates in treated hyphae compared to the untreated culture was remarkably shown. The mode of action of in vitro experiment manifested that AFP was effective to act the fungal cell and permeabilize the cell membrane of A. alternata. Furthermore, the in vivo experiment showed that AFP could reduce post-harvest decay on banana caused by A. alternata. AFP at concentration of 15 and 25 μg·ml-1 exhibit Alternaria decayed reduction by 45.45% and 77.27%, respectively. While no Alternaria decayed area was observed when 50 μg·ml-1 was applied during the storage time. Quantification of DNA by species-specific PCR could exude a positive correlation between the DNA amount and decayed area. In conclusion, AFP can be efficiently used as a bio-preservative agent during storage and handling of banana fruits, and considered as an excellent biological alternative to combat secondary growth of filamentous fungi.
Highlights
Antimicrobial proteins and peptides have been isolated from a wide range of plant and micro-organisms species
In order to analyse the antifungal activity of the antifungal protein (AFP) produced by A. giganteus against A. alternata as plant pathogenic fungi and to utilize the AFP in a selected food stuff
The results indicated that when artificially infected-wounded bananas were sprayed with different AFP concentrations, the growth of A. alternata was partly or totally inhibited depends on the applied concentration, Figure 8 and Figure 9
Summary
Antimicrobial proteins and peptides have been isolated from a wide range of plant and micro-organisms species. More recently it has become increasingly clear that these types of antimicrobial peptides play an important role in food bio-preservatives. In this approach, many antimicrobial and antifungal peptides were isolated and successfully used to prevent some foods from the microbial and fungal deteriorations [1]. Among the antifungal proteins produced by filamentous fungi, the imperfect Ascomycetes A. giganteus is characterized by its ability to secret some antifungal peptides. Liu et al [2] purified α-Sarcin and AFP by chitin affinity column chromatography and gel filtration, and obtained 3.45 mg∙AFP∙l−1. A few studies were established for purification different antifungal peptides applying the isoelectric focusing procedure [9]-[11], where no one used this technique for purification of AFP from A. gigenteus so far
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