Bio-capture of Cr(VI) in a denitrification system: Electron competition, long-term performance, and microbial community evolution

Abstract

Chromium is widely applied in industries as an important metal resource, but the discharge of Cr(VI) containing wastewater leads to the loss of chromium resources. This study proposed a bio-capture process of chromium in a denitrification system. The bio-capture potentiality was explored by investigating the electron competition between Cr(VI) and nitrogen compounds reduction, the long-term bio-capture performance, and the microbial community evolution. In the competition utilization of electron donors, both NO3--N and NO2--N took precedence over Cr(VI), and NO2--N reduction was proved to be the rate-limiting step. Under the optimum conditions of 20 mg/L NO3--N and 6 h HRT, 99.95% of 30 mg/L Cr(VI) could be reduced, and 220980 μg Cr/g MLSS was captured by the biofilm, which was fixed in intercellular as Cr(III). Microbiological analysis confirmed that the bio-reduction of Cr(VI) and NO3--N was mediated by synergistic interactions of a series of dominant bacteria, including genera Acidovorax, Thermomonas, and Microbacterium, which contained both the denitrification genes (narG, narZ, nxrA, and nirK) and chromate reduction genes (chrA and chrR). This study proved the feasibility of chromium bio-capture in denitrification systems and provided a new perspective for the Cr(VI) pollution treatment.