Bio-augmentation of the filler-enhanced denitrifying sulfide removal process in expanded granular sludge bed reactors

Abstract

Sulfur- and nitrogen-containing organic industrial wastewaters, which primarily result from several processes, including pharmaceutical, slaughter, papermaking, and petrochemical processes, are typical examples of refractory wastewaters. To ensure resource utilization, sulfur compounds at high concentrations in such wastewaters can be converted to elemental sulfur through specific methods. Specifically, the denitrifying sulfide removal (DSR) process can be employed to effectively recover elemental sulfur via biological sulfide oxidation, and reportedly, bio-augmentation presents as an effective strategy by which the challenges that limit the application of the DSR process can be overcome. However, the bacterial loss resulting from microorganism activity inhibition owing to toxic effect of high sulfide concentration as well as the complexity of the organic matter (carbon source) in actual wastewater environments reduce the actual elemental sulfur production rate. In this regard, the bio-augmentation effect of adding fillers under complex carbon source conditions was studied. The structure and function of the microbial community on the surface of the fillers were also analysed to reveal the internal factors that contributed to the increased efficiency of elemental sulfur generation. The results obtained showed that relative to the control, elemental sulfur generation increased 1.5- and 2-fold following the addition of fillers and fillers with microbial inoculants, respectively. Further, in the reactor with the added filler, the dominant bacteria in the biofilm on the filler surface were Pseudomonas and Azoarcus, while in reactor with added fillers plus microbial inoculates, the dominant bacteria in the biofilm on the filler surface were Pseudomonas and Arcobacter. These findings indicated that bio-augmentation promoted the expression of sulfur oxidation functional genes. Furthermore, adding Pseudomonas sp. gs1 for bio-augmentation improved the impact load resistance of the biofilm on the surface of the filler, leading to the rapid restoration of the elemental sulfur generation rate after the impact.