Abstract
A field experiment was carried out at Ismailia Agricultural Research Station, Ismailia Governorate, Egypt, during the two successive seasons of 2017 and 2018 to study the impact of beneficial microorganisms Trichoderma harzianum (fungi), Streptomyces griseus (bacteria) and two common chemical fungicides (copper sulphate and difenoconazole) to controlling spearmint rust disease. The vegetative growth, chemical constituents, essential oil production and its components in spearmint (Mentha viridis L.) plants were evaluated. The obtained results concluded that the use of copper sulfate as a chemical fungicide and the two bioagents [Streptomyces griseus and Trichoderma harzianum] reduced the incidence and percentage of rust disease severity in compared to untreated plants (control). Similarly, the level of vegetative growth, essential oil percentage and yield and the percentage of minerals and total carbohydrates in herb as well as carvone content in essential oil were noticeably more in compared to the chemical fungicide (difenoconazole) and untreated plants (control). Thus, reduce theusing of chemical fungicides means reducing environmental risks.
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