Binjai (Mangifera caesia) leaf extract nanoemulsion as antibacterial Escherichia coli

Abstract

Indonesia is a tropical country with a high level of infectious disease.. One of the bacteria that often infects and causes diarrhea is Escherichia coli. E. coli infection can cause food poisoning and indigestion. Infectious diseases caused by bacteria can be treated by taking antibiotics. However, excessive and inappropriate use of antibiotics can lead to bacterial resistance.Binjai (Mangifera caesia) containing components of polyphenolic compounds such as mangiferin, catechins and epicatechins with bioactivity as antibacterial. The potency of the plant extract needs to be increased as a natural antibacterial to facilitate its absorption with nanoemulsion formulation. Research methods include the manufacture of extracts with ethanol solvent maceration method. Next will be nanoemulsion formulation, measurement of nanoemulsion size by PSA analysis, and determination of antibacterial in vitro. Binjai leaf (Mangifera caesia) extract nanoemulsion formulation obtained stable nanoemulsion. The droplet size obtained is below 200 nm. Inhibition of nanoemulsion on growth of E. coli, it was found that the inhibitory power of nanoemulsion was still less than the inhibitory power of binjai leaf extract. This is because in the nanoemulsion the concentration of the extract is only 5 m and 25 mg.