Binding Sites of Quinones in Photosynthetic Bacterial Reaction Centers Investigated by Light-Induced FTIR Difference Spectroscopy: Assignment of the Interactions of Each Carbonyl of QA in Rhodobacter sphaeroides Using Site-Specific 13C-Labeled Ubiquinone

Abstract

Light-induced QA-/QA FTIR difference spectra of the photoreduction of the primary quinone (QA) have been obtained for Rhodobacter sphaeroides reaction centers (RCs) reconstituted with ubiquinone (Q3) labeled selectively with 13C at the 1- or 4-position of the quinone ring, i.e., on either of the two carbonyls. The vibrational modes of the quinone in the QA site are compared to those in vitro. IR absorption spectra of films of the labeled quinones show that the two carbonyls contribute equally to the split C = O band at 1663-1650 cm-1. This splitting is assigned to the two different geometries of the methoxy group nearest to each carbonyl. The QA-/QA spectra of RCs reconstituted with either 13C1- or 13C4-labeled Q3 and with unlabeled Q3 as well as the double differences calculated from these spectra exhibit distinct isotopic shifts for the bands assigned to C = O and C = C vibrations of the neutral QA. For the unlabeled QA, these bands correspond to the bands at 1660, 1628, and 1601 cm-1 previously detected upon nonselective isotopic labeling [Breton, J., Burie, J.-R., Berthomieu, C., Berger, G., & Nabedryk, E. (1994) Biochemistry 33, 4953-4965]. The 1660-cm-1 band is unaffected upon selective labeling at C4 but shifts to approximately 1623 cm-1 upon 13C1 labeling, demonstrating that this band arises from the C1 carbonyl, proximal to the isoprenoid chain. The band at 1628 cm-1 shifts by 11 and 16 cm-1 upon 13C1 and 13C4 labeling, respectively, and is assigned to a C = C mode coupled to both carbonyls.(ABSTRACT TRUNCATED AT 250 WORDS)