Binding Properties of Odorant-Binding Protein 7 to Host Volatiles in Larvae of Spodoptera frugiperda.

Abstract

The chemosensory system is crucial during the growth and development of the moths. Spodoptera frugiperda (Lepidoptera: Noctuidae) is one of the most destructive insect pests. However, there is little functional research on odorant-binding proteins (OBPs) in the larval stage of S. frugiperda. Here, we obtained SfruOBP7 from transcriptomics and conducted the sequence analysis. We used quantitative real-time PCR to explore the expression profiles of SfruOBP7. The function identification showed that SfruOBP7 has a binding ability to 18 plant volatiles. Further molecular docking and site-directed mutant assay revealed that Lys45 and Phe110 were the key binding sites for SfruOBP7 interacting with linalool. In the behavior assays, linalool could attract the larvae, and dsOBP7-treated larvae lost their attraction to linalool. Our results help to reveal the essential molecular mechanism of the olfactory perception in the larvae and design an attractant based on the host volatiles.