Abstract
To date only planktonic bacteria have been shown to bind irreversibly to dialkyl carbamoyl chloride (DACC)-coated Cutimed Sorbact dressings. Therefore, this study was designed to determine whether bacterial biofilm bound to the DACC-coated dressing in vitro. Samples of DACC-coated dressings and uncoated control dressings (supplied by BSN medical Ltd, Hull) were placed in contact with plastic coverslips on which biofilms of either Pseudomonas aeruginosa or methicillin-resistant Staphylococcus aureus (MRSA) had been cultivated for 24 hours. Dressing samples were examined by scanning electron microscopy to detect the presence of biofilm. Pseudomonas aeruginosa biofilm bound avidly to both DACC-coated and uncoated dressing samples. MRSA bound more extensively to DACC-coated dressings than to uncoated samples. Biofilms of two different test bacteria bound to dressings in vitro with the DACC-coating on the dressings enhancing the binding of MRSA biofilm. This study was supported by BSN medical Ltd (Hull). The company had no influence on the experimental design or the interpretation of the results.
Highlights
The need to reduce wound bioburden has long been recognised.[1]
Dressing samples exposed to P. aeruginosa biofilm indicated rapid and extensive acquisition of biofilm (Fig. 4); the extent of biofilm associated with dialkyl carbamoyl chloride (DACC)‐uncoated (Fig. 4a and b) and DACC‐coated dressings (Fig.4c and d) showed no marked differences
In order to determine whether the bacterial cells attached to DACC‐coated dressings were present as planktonic cells or as biofilms, some images at higher magnification were collected from samples tested with each of P. aeruginosa and methicillin‐resistant Staphylococcus aureus (MRSA)
Summary
The need to reduce wound bioburden has long been recognised.[1]. using the ability of microbial species to bind to wound dressings is a relatively recent approach to wound management that provides an antimicrobial effect without the use of an active inhibitory agent or the risk of cytotoxicity to host tissues. It was seen that biofilm transferred from the plastic coverslips directly to dressing samples (Fig. 3a) and did not migrate extensively through the dressing sample to the distal surface during the contact times tested here (up to 3 hours) (Fig. 3b).
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