Abstract
The binding of crotonase (enoyl-CoA hydratase), beta-hydroxyacyl-CoA dehydrogenase, beta-ketothiolase, succinyl-CoA transferase, and carnitine acetyltransferase to inner mitochondrial membranes, mitoplasts, intact mitochondria, erythrocyte membranes, and phosphatidylcholine liposomes was studied. Succinyl-CoA transferase does not bind to any of these membranes. Carnitine acetyltransferase, on the other hand, binds to all of these membranes. The enzymes of fatty acid beta-oxidation, thiolase, crotonase, and beta-hydroxyacyl-CoA dehydrogenase bind to inner membrane, but not to liposomes. The binding shows a moderate dependence on ionic strength (2-200 mM) and pH (6.5-8). These data indicate the possibility of an organization of the enzymes of fatty acid beta-oxidation on the inner mitochondrial membrane, but do not support the idea of an organization of the enzymes of ketone body catabolism.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
