Abstract
Equilibrium and kinetic studies of binding of proflavine and ethidium bromide to T2 phage indicate that (1) most of the intraphage DNA is accessible to proflavine; (2) the bulkier ethidium cation binds to a limited amount of DNA within the phage head; (3) intraphage DNA and free DNA in solutions of high ionic strength have similar equilibrium constants for binding to proflavine and ethidium; (4) on a time scale of minutes to hours, there is little difference in the permeabilities of the fast sedimenting (1000 S) and slow sedimenting (700S) forms of T2 phage as measured by proflavine binding.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Biochemical and Biophysical Research Communications
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.