Abstract
Particulate fractions of rat testis homogenate were prepared which bound 125I-labeled human pituitary LH (Hartree). Binding was competitively inhibited by nonlabeled pituitary human LH, HCG, and urinary HMG but not by human FSH. Effects of temperature, amount of binding preparation used, length of incubation, and size of tracer on LH binding were evaluated. Dose-response curves for pituitary LH, HCG, and urinary HMG are presented. In our system 1 mU of HCG has potency equal to about 1.2 ng of pituitary LH and to about 2.2 mIU of HMG. Comparison of Hartree LH and second IRP-HMG in the radioligand and radioimmunoassay systems gave equipotency figures of 1 ng of pituitary LH:2 mIU HMG in the ligand assay, and 1 ng of pituitary LH:8 mIU HMG in the immunoassay system. This “immuno:ligand ratio” of four for pituitary LH, when using a urinary HMG standard, is similar to reported “immunobioassay ratios” employing classical bioassay methods.
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