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Abstract
Giant unilamellar vesicles (GUVs) are model systems for biomembranes around cells and organelles. Peripheral proteins on cellular membranes are important for signaling and membrane trafficking. The binding of peripheral proteins to the GUV membrane can be achieved by direct interaction with specific lipids. Histidine tag molecules attach to 1,2-dioleoyl-sn-glycero-3-[(N-(5-amino-1-carboxypentyl)iminodiacetic acid)succinyl] (nickel salt) DGS-NTA (Ni) lipids forming an octahedral co-ordinate complex.
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