Binding of germanium and lead to Pseudomonas putida lipopolysaccharides

Abstract

The binding of germanium and lead ions to lipopolysaccharide isolated from Pseudomonas putida ATCC 33015 was studied by high-resolution 31P nuclear magnetic resonance spectroscopy. Chemical shift changes toward higher field as well as broadening of all phosphorus resonances were observed with added lead. Addition of germanium to the lipopolysaccharide sample resulted in more pronounced chemical shift changes toward lower field. The effects of binding of germanium and lead ions on the 31P nuclear magnetic resonance spectrum of lipopolysaccharide imply that the glycosidic monophosphate joined to the reducing end of N-acetylglucosamine (C-1 position of α-D-G1cNI) as well as β-phosphate groups of glycosidically linked diphosphates of the same residue of the lipid A moiety provide high-affinity metal-binding sites.Key words: germanium, lead, lipopolisaccharide (LPS), binding, Pseudomonas putida.