Binding of crystal violet by nucleic acids of Escherichia coli.

Abstract

Abstract The absorption isotherms of DNA and RNA from E. coli for crystal violet are sigmoid. Binding of dye was determined i) by difference in its absorbance before and after addition of nucleic acids and ii) by measuring the amount of dye dialysing through a membrane containing dye and nucleic acid. pH change caused no significant change in the degree of binding.